Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
The Oser Laboratory in the Division of Molecular and Cellular Oncology and the Department of Medical Oncology at the Dana-Farber Cancer Institute/Harvard Medical School focuses on identifying new therapeutic targets in small cell lung cancer (SCLC).
SCLC is a highly aggressive neuroendocrine cancer that is driven by loss of function (LOF) mutations in the tumor suppressor genes RB1, TP53, and NOTCH. It is also characterized by high expression of oncogenic neural/neuroendocrine transcription factors including ASCL1, NEUROD1, and POU2F3, which are dependencies in SCLC, but are undruggable. Our lab utilizes several approaches including CRISPR/Cas9-based functional screening and developing genetically-engineered mouse models of SCLC using CRISPR/Cas9 to identify novel ways to target LOF mutations in tumor suppressor genes and undruggable oncogenic drivers. Through our research, we seek to gain a more comprehensive understanding of SCLC tumorigenesis and identify new targeted therapy approaches for patients with SCLC.
Dr. Oser is seeking a highly motivated laboratory research technician to join a research team that works to identify novel therapeutic targets in small cell lung cancer using both in vitro and in vivo approaches. This individual will work with the principal investigator to perform cutting-edge research with potential impact on cancer treatment. The ability to perform a variety of cellular and molecular biology techniques is essential for this role, as are good organizational and communication skills, ability to work within a team, flexibility and responsibility.
The position will require a core foundation of molecular and cellular biology with some experience in tissue culture, and willingness to work with mice.
The successful candidate will be expected to help with several projects and will be performing the following techniques/tasks.
Specific duties would include:
- Extraction and analysis of DNA and mRNA, including PCR, quantitative PCR, RT-PCR, and CRISRP amplicon sequencing.
-Cloning using restriction enzymes and recombination cloning.
-Generation of lentiviruses and adenoviruses.
-Protein chemical assays including immunoblotting.
-Transfection and transduction of mammalian cell lines
-Cell proliferation assays following pharmacological treatments and genetic inactivation by CRISPR.
-Mouse colony breeding and maintenance.
-Data analysis and recording of results electronically and in a lab notebook.
-Working closely with a scientist who will serve as direct supervisor and mentor.
-Ordering lab supplies
-Preparation of laboratory reagents
We are looking for an enthusiastic, capable, and competent scientist, with a BA/BS in biology or a related field. Preference will be given to those with a strong background in coursework in molecular and cellular biology with at least some laboratory experience in that area. In addition to technical and scientific knowledge, this individual should have good organizational and interpersonal skills and a good problem solver. Strong preference will be given to individuals that will spend 2 years as a technician and are highly motivated to go on to pursue either an MD, PhD, or MD/PhD following this position.
Full-time, 40 hours.
Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.
Interested applicants can email Dr. Oser directly (firstname.lastname@example.org) with their CV.